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GrainGenes Marker Report: Xcfd62

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Locus	Xcfd62
Type	Microsatellite
Map	Wheat, Avalon x Cadenza 3D 0.3 Wheat, UC1110 x PI610750 3D 41.1
	[ Show Nearby Loci ]
Map Data	Wheat, Avalon x Cadenza Wheat, UC1110 x PI610750
Probe	CFD62
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xcfd62-2D [ Show all 12 ]
Probe for Locus	CFD62
Locus	Xcfd62.1-2D [ Show all 13 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)27 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CAAGAGCTGACCAATGTGGA 3' Reverse 5' ACGGCGGTGAGATGAG 3'
SSR size	220
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D/7A
Source Species	Aegilops tauschii