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GrainGenes Marker Report: Xcfd61-1D

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Locus	Xcfd61-1D
Type	Microsatellite
Chromosome	1D
Map	Ta-Courtot/CS-GPW-1D 27.3 Ta-SSR-2004-1D 26.4
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW
Probe	CFD61
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd61 cfd61 cfd61-1D
Probe for Locus	CFD61
Locus	Xcfd61-1D Xcfd61 cfd61 cfd61-1D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CACAA)3(AC)22 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' ATTCAAATGCAACGCAAACA 3' Reverse 5' GTTAGCCAAGGACCCCTTTC 3'
SSR size	218
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1D
Source Species	Aegilops tauschii