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GrainGenes Marker Report: Xcfd6-7A

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Locus	Xcfd6-7A
Type	Microsatellite
Chromosome	7A
Map	Kofa/UC1113-7A 171.2 Ta-Courtot/CS-GPW-7A 129.5 Ta-SSR-2004-7A 66.6
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 Durum wheat, Svevo x Ciccio Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW
Probe	CFD6
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	cfd6 [ Show all 9 ]
Probe for Locus	CFD6
Locus	Xcfd6-7A [ Show all 10 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)6(GCTA)4 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' ACTCTCCCCCTCGTTGCTAT 3' Reverse 5' ATTTAAGGGAGACATCGGGC 3'
SSR size	236
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7A
Source Species	Aegilops tauschii