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GrainGenes Marker Report: Xcfd57-5D

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Locus	Xcfd57-5D
Type	Microsatellite
Chromosome	5D
Map	Conan/Reeder-5D 72.8804 CS/SQ1-5D 163.1 Louise/Penawawa-5D 113.3 Ta-Courtot/CS-GPW-5D 139.4 Ta-SSR-2004-5D 56.2
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa
Probe	CFD57
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd57 cfd57-5D cfd57
Probe for Locus	CFD57
Locus	Xcfd57-5D Xcfd57 cfd57-5D cfd57
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)29 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' ATCGCCGTTAACATAGGCAG 3' Reverse 5' TCACTGCTGTATTTGCTCCG 3'
SSR size	291
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii