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GrainGenes Marker Report: Xcfd54a

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Locus	Xcfd54a
Type	Microsatellite
Chromosome	4D
Map	Wheat-Composite2004-4D 93
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Map Data	Wheat, Composite, 2004
Probe	CFD54
Possible Orthologs	Xcfd54-4B Xcfd54-4D cfd54 cfd54-4B
Probe for Locus	CFD54
Locus	Xcfd54-4B Xcfd54-4D Xcfd54a cfd54 cfd54-4B
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)42 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TCGTTCCAAAATGCATGAAA 3' Reverse 5' AAGGGCCAGAAATCTGTGTG 3'
SSR size	200
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4D/4B
Source Species	Aegilops tauschii