GrainGenes Marker Report: Xcfd53-2D
Locus
Xcfd53-2D
Type
Microsatellite
Chromosome
2D
Chromosome Arm
2DS
Map
Chinese_Spring_Deletion_SSR_2D -0.4 CS/SQ1-2D 22.5 Ta-Courtot/CS-GPW-2D 40 Triticum-AxF-2D 16.8 USG3209/Jaypee-2D 5.4
[ Show Nearby Loci ]
Breakpoint Interval
2DS1-0.33-0.47
Map Data
Wheat, Arina x Forno Wheat, Chinese Spring x SQ1 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR Wheat, USG3209 x Jaypee
Species
Triticum aestivum
Probe
CFD53
Reference
Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
Xcfd53
Probe for Locus
CFD53
Locus
Xcfd53-2D Xcfd53
Reference
General Remarks
Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)22(CA)10 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' CCCTATTTCCCCCATGTCTT 3'
Reverse 5' AAGGAGGGCACATATCGTTG 3'
SSR size
233
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd53-2D
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