GrainGenes Marker Report: Xcfd51-2D
Locus
Xcfd51-2D
Type
Microsatellite
Chromosome
2D
Map
Chinese_Spring_Deletion_SSR_2D -0.735 Louise/Penawawa-2D 132.8 Ta-Courtot/CS-GPW-2D 12.3 Ta-SSR-2004-2D 9.3
[ Show Nearby Loci ]
Breakpoint Interval
2DS5-0.47-1.00
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa Wheat, Physical, SSR
Probe
CFD51
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd51 Xcfd51a-2D
Probe for Locus
CFD51
Locus
Xcfd51a-2D Xcfd51-2D Xcfd51
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)45 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GGAGGCTTCTCTATGGGAGG 3'
Reverse 5' TGCATCTTATCCTGTGCAGC 3'
SSR size
172
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd51-2D
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