GrainGenes Marker Report: Xcfd51
Locus
Xcfd51
Type
Microsatellite
Chromosome
2D
Map
Ta-NxW-2D 11.6 Wheat, UC1110 x PI610750 2D 0 Wheat-2008-Corrigin-CS(T2D)-2D 16 Wheat-Composite2004-2D 36
[ Show Nearby Loci ]
Map Data
Wheat 2008, Corrigin x CS(T2D), C gene Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe
CFD51
Reference
Possible Orthologs
Xcfd51-2D Xcfd51a-2D
Probe for Locus
CFD51
Locus
Xcfd51a-2D Xcfd51-2D Xcfd51
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)45 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GGAGGCTTCTCTATGGGAGG 3'
Reverse 5' TGCATCTTATCCTGTGCAGC 3'
SSR size
172
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd51
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