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GrainGenes Marker Report: Xcfd47-6B

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Locus	Xcfd47-6B
Type	Microsatellite
Map	USG3209/Jaypee-6B 225.2
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Map Data	Wheat, USG3209 x Jaypee
Probe	CFD47
Possible Orthologs	Xcfd47 Xcfd47-5A Xcfd47-6D
Probe for Locus	CFD47
Locus	Xcfd47-6D Xcfd47-6B Xcfd47 Xcfd47-5A
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. SrTA10187 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GT)25 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TGACCATGTCATGTTTTATACCACT 3' Reverse 5' TGGACTACATGTCAAGCACAAA 3'
SSR size	184
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6D
Source Gene	SrTA10187
Source Species	Aegilops tauschii