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GrainGenes Marker Report: Xcfd44

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Locus
Xcfd44
Type
Microsatellite
Chromosome
2D
Map
Wheat, Avalon x Cadenza 2D122.6
Wheat-2008-Corrigin-CS(T2D)-2D126
Wheat-Composite2004-2D90
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Map Data
Wheat 2008, Corrigin x CS(T2D), C gene
Wheat, Avalon x Cadenza
Wheat, Composite, 2004
Probe
CFD44
Possible Orthologs
Xcfd44-2D
cfd44



Probe for Locus
CFD44
Locus
Xcfd44-2D
Xcfd44
cfd44
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = D
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CT)24
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' AAACCCAATGGCTCTCACAC 3'
Reverse 5' ATGGCCCAATTATGCAACTC 3'
SSR size
238
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii