GrainGenes Marker Report: Xcfd41-7D
Locus
Xcfd41-7D
Type
Microsatellite
Chromosome
7D
Map
Chinese_Spring_Deletion_SSR_7D -0.18 Louise/Penawawa-7D 26.8 Ta-Courtot/CS-GPW-7D 35.3 Ta-SSR-2004-7D 44
[ Show Nearby Loci ]
Breakpoint Interval
C-7DS5-0.36
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa Wheat, Physical, SSR
Probe
CFD41
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd41 cfd41
Probe for Locus
CFD41
Locus
Xcfd41-7D Xcfd41 cfd41
Reference
General Remarks
Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)17 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TAAAGTCTCAGGCGACCCAC 3'
Reverse 5' AGTGATAGACGGATGGCACC 3'
SSR size
286
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd41-7D
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