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GrainGenes Marker Report: Xcfd39b

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Locus	Xcfd39b
Type	Microsatellite
Chromosome	4D 5A
Map	Wheat-Composite2004-4D 129 Wheat-Composite2004-5A 83
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Map Data	Wheat, Composite, 2004
Probe	CFD39
Possible Orthologs	Xcfd39 [ Show all 13 ]
Probe for Locus	CFD39
Locus	Xcfd39-4B [ Show all 14 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CCACAGCTACATCATCTTTCCTT 3' Reverse 5' CAAAGTTTGAACAGCAGCCA 3'
SSR size	175
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4B/5A/4D
Source Species	Aegilops tauschii