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GrainGenes Marker Report: Xcfd36-2D

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Locus	Xcfd36-2D
Type	Microsatellite
Chromosome	2D
Map	Chinese_Spring_Deletion_SSR_2D -0.735 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	2DS5-0.47-1.00
Map Data	Wheat, Consensus SSR, 2004 [ Show all 6 ]
Probe	CFD36
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd36 Xcfd36-2A Xcfd36b cfd36 cfd36-2A
Probe for Locus	CFD36
Locus	Xcfd36-2D [ Show all 6 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)25(CCCG)3 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GCAAAGTGTAGCCGAGGAAG 3' Reverse 5' TTAGAGTTTTGCAGCGCCTT 3'
SSR size	192
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D/2A
Source Species	Aegilops tauschii