GrainGenes Marker Report: Xcfd33-6D
Locus
Xcfd33-6D
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_6D -0.89 Louise/Penawawa-6D 86.3 P91193D1/P92201D5-6D 0 Ta-Courtot/CS-GPW-6D 0
[ Show Nearby Loci ]
Breakpoint Interval
6DS4-0.79-0.99
Map Data
Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR
Probe
CFD33
Possible Orthologs
Xcfd33 cfd33-6D
Probe for Locus
CFD33
Locus
Xcfd33-6D Xcfd33 cfd33-6D
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)18 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' TACCGCAATAATCACACCCA 3'
Reverse 5' GGTCGATGGACTGTCCCTAA 3'
SSR size
170
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
6D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd33-6D
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