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GrainGenes Marker Report: Xcfd3

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Locus	Xcfd3
Type	Microsatellite
Chromosome	5D
Map	Wheat-Composite2004-5D 52
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Map Data	Wheat, Composite, 2004
Probe	CFD3
Possible Orthologs	Xcfd3-5D cfd3
Probe for Locus	CFD3
Locus	Xcfd3-5D Xcfd3 cfd3
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)16 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GCACCAACACACGGAGAAG 3' Reverse 5' TTGAGAGGAGGGCTTGGTTA 3'
SSR size	163
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii