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GrainGenes Marker Report: Xcfd2a

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Locus	Xcfd2a
Type	Microsatellite
Chromosome	3A 5B
Map	Wheat-Composite2004-3A 40 Wheat-Composite2004-5B 57
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Map Data	Wheat, Composite, 2004
Probe	CFD2
Possible Orthologs	Xcfd2 [ Show all 27 ]
Probe for Locus	CFD2
Locus	Xcfd2-2A [ Show all 28 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)21 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GGTTGCAGTTTCCACCTTGT 3' Reverse 5' CATCTATTGCCAAAATCGCA 3'
SSR size	288
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2A/2D/3A/3D/4A/5B/5D
Source Species	Aegilops tauschii