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GrainGenes Marker Report: Xcfd28-3B

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Locus	Xcfd28-3B
Type	Microsatellite
Chromosome	3B
Map	Ta-SSR-2004-3B 34.9
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Map Data	Wheat, Consensus SSR, 2004
Probe	CFD28
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd28 Xcfd28-1D cfd28 cfd28-3B
Probe for Locus	CFD28
Locus	Xcfd28-1D Xcfd28 Xcfd28-3B cfd28 cfd28-3B
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CAA)22 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TGCATCTTATTACTGGAGGCATT 3' Reverse 5' CGCATGCCCTTATACCAACT 3'
SSR size	190
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1D
Source Species	Aegilops tauschii