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GrainGenes Marker Report: Xcfd28-1D

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Locus	Xcfd28-1D
Type	Microsatellite
Map	P91193D1/P92201D5-1D 9.9 Ta-Courtot/CS-GPW-1D 45.3
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Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, P91193D1 x P92201D5
Probe	CFD28
Possible Orthologs	Xcfd28 Xcfd28-3B cfd28 cfd28-3B
Probe for Locus	CFD28
Locus	Xcfd28-1D Xcfd28 Xcfd28-3B cfd28 cfd28-3B
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CAA)22 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TGCATCTTATTACTGGAGGCATT 3' Reverse 5' CGCATGCCCTTATACCAACT 3'
SSR size	190
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1D
Source Species	Aegilops tauschii