GrainGenes Marker Report: Xcfd26-5D
Locus
Xcfd26-5D
Type
Microsatellite
Chromosome
5D
Chromosome Arm
5DL
Map
Chinese_Spring_Deletion_SSR_5D 0.75 Ta-Courtot/CS-GPW-5D 142.8 Ta-SSR-2004-5D 50.8 Triticum-AxF-5D 96.5
[ Show Nearby Loci ]
Breakpoint Interval
5DL9-0.74-0.76
Map Data
Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Species
Triticum aestivum
Probe
CFD26
Reference
Possible Orthologs
Xcfd26 Xcfd26-2A Xcfd26-7D cfd26 cfd26-5D
Probe for Locus
CFD26
Locus
Xcfd26-5D
[ Show all 7 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GAGAA)2(GA)37 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TCAAGATCGTGCCAAATCAA 3'
Reverse 5' ACTCCAAGCTGAGCACGTTT 3'
SSR size
271
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd26-5D
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