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GrainGenes Marker Report: Xcfd25a

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Locus	Xcfd25a
Type	Microsatellite
Chromosome	7D
Map	Wheat-Composite2004-7D 159
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD25
Possible Orthologs	Xcfd25 Xcfd25-2B Xcfd25-5D Xcfd25-7D Xcfd25b
Probe for Locus	CFD25
Locus	Xcfd25-2B [ Show all 6 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)35 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CATCGCTCATGCTAAGGTCA 3' Reverse 5' CGTGTCTGTTAGCTGGGTGG 3'
SSR size	203
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2B/7D/5D
Source Species	Aegilops tauschii