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GrainGenes Marker Report: Xcfd257

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Locus	Xcfd257
Type	Microsatellite
Chromosome	4A
Map	Wheat-Composite2004-4A 92
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Map Data	Wheat, Composite, 2004
Probe	CFD257
Possible Orthologs	Xcfd257-4A cfd257
Probe for Locus	CFD257
Locus	Xcfd257-4A Xcfd257 cfd257
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GAA)6 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TCTCAACTTGCAACTGCCAC 3' Reverse 5' CCCTCCATGGATTCTTGCTA 3'
SSR size	287
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4A
Source Species	Aegilops tauschii