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GrainGenes Marker Report: Xcfd255-2D

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Locus	Xcfd255-2D
Type	Microsatellite
Map	Ta-Synthetic/Opata-GPW-2D 109
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Map Data	Wheat, Synthetic x Opata, GPW
Probe	CFD255
Possible Orthologs	Xcfd255
Probe for Locus	CFD255
Locus	Xcfd255-2D Xcfd255
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)7 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' AAGTCGACAGGTCGGTTGAG 3' Reverse 5' TTTACGCAGGATACGGCTCT 3'
SSR size	235
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D
Source Species	Aegilops tauschii