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GrainGenes Marker Report: Xcfd24-4A

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Locus	Xcfd24-4A
Type	Microsatellite
Map	CS/SQ1-4A 170.6 Ta-Courtot/CS-GPW-4A 37.2
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Courtot x Chinese Spring, GPW
Probe	CFD24
Probe for Locus	CFD24
Locus	Xcfd24-4A
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (A)n(T)n Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GGCGCAATCTGAAAGAAAAG 3' Reverse 5' CCAGGTCCCACTTTCGTCT 3'
SSR size	273
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4A
Source Species	Aegilops tauschii