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GrainGenes Marker Report: Xcfd238-2B

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Locus	Xcfd238-2B
Type	Microsatellite
Map	Louise/Penawawa-2B 116.1
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Map Data	Wheat, Louise x Penawawa
Probe	CFD238
Possible Orthologs	Xcfd238 cfd238
Probe for Locus	CFD238
Locus	Xcfd238-2B Xcfd238 cfd238
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GGGA)3 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GTTGAGGAGGACAAAGAGGC 3' Reverse 5' GATACGAGCGAGCCCATAAA 3'
SSR size	151
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2B
Source Species	Aegilops tauschii