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GrainGenes Marker Report: Xcfd233-2D

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Locus	Xcfd233-2D
Type	Microsatellite
Chromosome	2D
Map	Chinese_Spring_Deletion_SSR_2D 0.245 Ta-SSR-2004-2D 78.7 Ta-Synthetic/Opata-GPW-2D 151.3
	[ Show Nearby Loci ]
Breakpoint Interval	C-2DL3-0.49
Map Data	Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe	CFD233
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd233 cfd233
Probe for Locus	CFD233
Locus	Xcfd233-2D Xcfd233 cfd233
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)38 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GAATTTTTGGTGGCCTGTGT 3' Reverse 5' ATCACTGCACCGACTTTTGG 3'
SSR size	289
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D
Source Species	Aegilops tauschii