Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xcfd23

[Submit comment/correction]

Locus	Xcfd23
Type	Microsatellite
Chromosome	4D
Map	Wheat-Composite2004-4D 45
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD23
Possible Orthologs	cfd23 cfd023
Probe for Locus	CFD23
Locus	Xcfd23-4D Xcfd23 Lr67/Yr46/Sr55/Pm46/Ltn3 cfd23 cfd023
Reference	Hiebert CW et al. (2010) An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67) Theoretical and Applied Genetics 121:1083-1091. Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172. Hiebert CW MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Leaf Rust Resistance. Lr67 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (AGT)23(GCA)3(GTA)8 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TAGCAGTAGCAGCAGCAGGA 3' Reverse 5' GCAAGGAAGAGTGTTCAGCC 3'
SSR size	187
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4D
Source Species	Aegilops tauschii