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GrainGenes Marker Report: Xcfd213-6D

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Locus	Xcfd213-6D
Type	Microsatellite
Map	Ta-Synthetic/Opata-GPW-6D 34.6
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Map Data	Wheat, Synthetic x Opata, GPW
Probe	CFD213
Possible Orthologs	Xcfd213-3D Xcfd213a Xcfd213b
Probe for Locus	CFD213
Locus	Xcfd213-3D Xcfd213-6D Xcfd213a Xcfd213b
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CCG)5 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GAGGTGGTTGCTGCCTAGAT 3' Reverse 5' TGCAAGGACCGAATAAGAGG 3'
SSR size	229
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D/6D
Source Species	Aegilops tauschii