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GrainGenes Marker Report: Xcfd19-6D.2

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Locus	Xcfd19-6D.2
Type	Microsatellite
Chromosome	6D
Chromosome Arm	6DS
Map	Triticum-AxF-6D 95.1
	[ Show Nearby Loci ]
Map Data	Wheat, Arina x Forno
Species	Triticum aestivum
Probe	CFD19
Reference	Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	Xcfd19 [ Show all 11 ]
Probe for Locus	CFD19
Locus	Xcfd19-1D [ Show all 12 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)18 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TACGCAGGTTTGCTGCTTCT 3' Reverse 5' GGAGTTCACAAGCATGGGTT 3'
SSR size	267
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1D/5D/6D
Source Species	Aegilops tauschii