GrainGenes Marker Report: Xcfd19-6D
Locus
Xcfd19-6D
Type
Microsatellite
Chromosome
6D
Map
Chinese_Spring_Deletion_SSR_6D -0.225 Ta-Courtot/CS-GPW-6D 16 Ta-SSR-2004-6D 49.7 Ta-Synthetic/Opata-GPW-6D 71
[ Show Nearby Loci ]
Breakpoint Interval
C-6DS2-0.45
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFD19
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd19
[ Show all 11 ]
Probe for Locus
CFD19
Locus
Xcfd19-1D
[ Show all 12 ]
Reference
General Remarks
Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)18 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TACGCAGGTTTGCTGCTTCT 3'
Reverse 5' GGAGTTCACAAGCATGGGTT 3'
SSR size
267
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1D/5D/6D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd19-6D
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|