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GrainGenes Marker Report: Xcfd170-1B

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Locus	Xcfd170-1B
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_1B -0.67 Ta-Synthetic/Opata-GPW-1B 30.3
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Breakpoint Interval	1BS10-0.50-0.84
Map Data	Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe	CFD170
Probe for Locus	CFD170
Locus	Xcfd170-1B
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GGC)4 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CTGTCGGACGACGACGA 3' Reverse 5' CATCCTCTTGACGCCGCCGC 3'
SSR size	268
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1B
Source Species	Aegilops tauschii