GrainGenes Marker Report: Xcfd168-2D
Locus
Xcfd168-2D
Type
Microsatellite
Chromosome
2D
Map
Chinese_Spring_Deletion_SSR_2D 0.88 Louise/Penawawa-2D 3.6 Ta-SSR-2004-2D 88.4 Wheat-2008-Corrigin-CS(T2D)-2D 113
[ Show Nearby Loci ]
Breakpoint Interval
2DL9-0.76-1.00
Map Data
Wheat 2008, Corrigin x CS(T2D), C gene Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, Physical, SSR
Probe
CFD168
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd168
[ Show all 8 ]
Probe for Locus
CFD168
Locus
Xcfd168-2D.1
[ Show all 9 ]
Reference
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CTG)20 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CTTCGCAAATCGAGGATGAT 3'
Reverse 5' TTCACGCCCAGTATTAAGGC 3'
SSR size
197
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd168-2D
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|