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GrainGenes Marker Report: Xcfd160-2D

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Locus	Xcfd160-2D
Type	Microsatellite
Chromosome	2D
Map	Ta-SSR-2004-2D 68.8 Ta-Synthetic/Opata-GPW-2D 122.7
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW
Probe	CFD160
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd160a cfd160
Probe for Locus	CFD160
Locus	Xcfd160-2D Xcfd160a cfd160
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CCCT)6 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CCACTACTGCGGCTAGGTCT 3' Reverse 5' CTTTTCCGTGTCTCCCTAGC 3'
SSR size	200
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D
Source Species	Aegilops tauschii