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GrainGenes Marker Report: Xcfd156

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Locus	Xcfd156
Type	Microsatellite
Chromosome	5B
Map	Wheat-Composite2004-5B 87
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Map Data	Wheat, Composite, 2004
Probe	CFD156
Possible Orthologs	Xcfd156-5B Xcfd156-5D cfd156 cfd156-5D
Probe for Locus	CFD156
Locus	Xcfd156-5B Xcfd156 Xcfd156-5D cfd156 cfd156-5D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CACGCA)3 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' AGCAGTGTAATAAAAGGGCG 3' Reverse 5' GTATTCGCACCAGAATCCGT 3'
SSR size	300
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5B
Source Species	Aegilops tauschii