GrainGenes Marker Report: Xcfd15-1D
Locus
Xcfd15-1D
Type
Microsatellite
Chromosome
1D
Chromosome Arm
1DS
Map
Chinese_Spring_Deletion_SSR_1D -0.24 Ta-SSR-2004-1D 23.2 Ta-Synthetic/Opata-GPW-1D 28.1 Triticum-AxF-1D 19.5
[ Show Nearby Loci ]
Breakpoint Interval
C-1DS3-0.48
Map Data
Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Species
Triticum aestivum
Probe
CFD15
Reference
Possible Orthologs
Xcfd15
[ Show all 9 ]
Probe for Locus
CFD15
Locus
Xcfd15-1A
[ Show all 11 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)20(TGTA)3 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' CTCCCGTATTGAGCAGGAAG 3'
Reverse 5' GGCAGGTGTGGTGATGATCT 3'
SSR size
179
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A/1D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd15-1D
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