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GrainGenes Marker Report: Xcfd141-3D

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Locus	Xcfd141-3D
Type	Microsatellite
Chromosome	3D
Chromosome Arm	3DS
Map	Chinese_Spring_Deletion_SSR_3D -0.775 Ta-Synthetic/Opata-GPW-3D 20.9 Triticum-AxF-3D 23.7
	[ Show Nearby Loci ]
Breakpoint Interval	3DS6-0.55-1.00
Map Data	Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Species	Triticum aestivum
Probe	CFD141
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	Xcfd141
Probe for Locus	CFD141
Locus	Xcfd141-3D Xcfd141
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TCAA)4 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CGTAAAGATCCGAGAGGGTG 3' Reverse 5' TCCGAGGTGCTACCTACCAG 3'
SSR size	155
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D
Source Species	Aegilops tauschii