GrainGenes Marker Report: Xcfd13-6D
Locus
Xcfd13-6D
Type
Microsatellite
Chromosome
6D
Chromosome Arm
6DS
Map
Chinese_Spring_Deletion_SSR_6D -0.995 Ta-SSR-2004-6D 21.3 Ta-Synthetic/Opata-GPW-6D 23.9 Triticum-AxF-6D 41.8
[ Show Nearby Loci ]
Breakpoint Interval
6DS6-0.99-1.00
Map Data
Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Species
Triticum aestivum
Probe
CFD13
Reference
Possible Orthologs
Xcfd13
[ Show all 14 ]
Probe for Locus
CFD13
Locus
Xcfd13-6B
[ Show all 16 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)20(TGTA)3 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CCACTAACCAAGCTGCCATT 3'
Reverse 5' TTTTTGGCATTGATCTGCTG 3'
SSR size
254
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
6B/6D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd13-6D
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