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GrainGenes Marker Report: Xcfd13-6B.2

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Locus	Xcfd13-6B.2
Type	Microsatellite
Chromosome	6B
Chromosome Arm	6BS
Map	Triticum-AxF-6B 6.2
	[ Show Nearby Loci ]
Map Data	Wheat, Arina x Forno
Species	Triticum aestivum
Probe	CFD13
Reference	Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Probe for Locus	CFD13
Locus	Xcfd13-6B [ Show all 16 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CT)20(TGTA)3 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CCACTAACCAAGCTGCCATT 3' Reverse 5' TTTTTGGCATTGATCTGCTG 3'
SSR size	254
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6B/6D
Source Species	Aegilops tauschii