GrainGenes Marker Report: Xcfd12-5D
Locus
Xcfd12-5D
Type
Microsatellite
Chromosome
5D
Map
Chinese_Spring_Deletion_SSR_5D 0.67 Ta-Courtot/CS-GPW-5D 163.2 Ta-SSR-2004-5D 54.9 Ta-Synthetic/Opata-GPW-5D 84.3
[ Show Nearby Loci ]
Breakpoint Interval
5DL1-0.60-0.74
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFD12
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd12 Xcfd12-5A cfd12 cfd12-5D
Probe for Locus
CFD12
Locus
Xcfd12-5D Xcfd12 Xcfd12-5A cfd12 cfd12-5D
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CTT)6 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' GTTACCCAAACCTGCCCTTT 3'
Reverse 5' CTACGAGTCGGGATCAGCAT 3'
SSR size
192
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd12-5D
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|