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GrainGenes Marker Report: Xcfd11a

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Locus	Xcfd11a
Type	Microsatellite
Chromosome	2D
Map	Wheat-Composite2004-2D 18
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD11
Possible Orthologs	Xcfd11-2B Xcfd11-2D Xcfd11.1 Xcfd11.2 Xcfd11b
Probe for Locus	CFD11
Locus	Xcfd11-2D [ Show all 6 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)31 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GGATTTGCCTTGGATTTGAA 3' Reverse 5' TTGCATCTCACGCACCTAAG 3'
SSR size	167
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D/2B
Source Species	Aegilops tauschii