GrainGenes Marker Report: Xcfd116-2D
Locus
Xcfd116-2D
Type
Microsatellite
Chromosome
2D
Map
Chinese_Spring_Deletion_SSR_2D -0.165 Louise/Penawawa-2D 41.3 Ta-SSR-2004-2D 65.8 Ta-Synthetic/Opata-GPW-2D 125.3
[ Show Nearby Loci ]
Breakpoint Interval
C-2DS1-0.33
Map Data
Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFD116
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfd116 cfd116
Probe for Locus
CFD116
Locus
Xcfd116-2D Xcfd116 cfd116
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)15 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' TTTGCCCATTACAACAAGCA 3'
Reverse 5' CAAGCAGCACCTCATGACAG 3'
SSR size
225
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd116-2D
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