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GrainGenes Marker Report: Xcfd116

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Locus	Xcfd116
Type	Microsatellite
Chromosome	2D
Map	Wheat-2008-Corrigin-CS(T2D)-2D 79 Wheat-Composite2004-2D 38
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Map Data	Wheat 2008, Corrigin x CS(T2D), C gene Wheat, Composite, 2004
Probe	CFD116
Possible Orthologs	Xcfd116-2D cfd116
Probe for Locus	CFD116
Locus	Xcfd116-2D Xcfd116 cfd116
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)15 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TTTGCCCATTACAACAAGCA 3' Reverse 5' CAAGCAGCACCTCATGACAG 3'
SSR size	225
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2D
Source Species	Aegilops tauschii