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GrainGenes Marker Report: Xcfd101-5D

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Locus	Xcfd101-5D
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_5D 0.67 Ta-Synthetic/Opata-GPW-5D 86.5
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Breakpoint Interval	5DL1-0.60-0.74
Map Data	Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe	CFD101
Possible Orthologs	Xcfd101
Probe for Locus	CFD101
Locus	Xcfd101-5D Xcfd101
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CGG)5 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCGACCGAAGAGAGCAGAAT 3' Reverse 5' AGAATGCGTCCTTTTCTTGC 3'
SSR size	299
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii