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GrainGenes Marker Report: Xcfd0223-3D

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Locus	Xcfd0223-3D
Type	Microsatellite
Map	P91193D1/P92201D5-3D 157.7
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Map Data	Wheat, P91193D1 x P92201D5
Probe	CFD223
Possible Orthologs	Xcfd23-4D Xcfd223 Xcfd223-2A Xcfd223-3D
Probe for Locus	CFD223
Locus	Xcfd223-3D Xcfd223 Xcfd0223-3D Xcfd223-2A
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)18 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' AAGAGCTACAATGACCAGCAGA 3' Reverse 5' GCAGTGTATGTCAGGAGAAGCA 3'
SSR size	153
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D
Source Species	Aegilops tauschii