GrainGenes Marker Report: Xcfa2278-2B
Locus
Xcfa2278-2B
Type
Microsatellite
Chromosome
2B
Map
Chinese_Spring_Deletion_SSR_2B 0.18 Conan/Reeder-2B 114.238 Ta-SSR-2004-2B 62.1 Ta-Synthetic/Opata-GPW-2B 72.7
[ Show Nearby Loci ]
Breakpoint Interval
C-2BL2-0.36
Map Data
Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFA2278
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2278 cfa2278
Probe for Locus
CFA2278
Locus
Xcfa2278-2B Xcfa2278 cfa2278
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GCG)8 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GCCTCTGCAAGTCTTTACCG 3'
Reverse 5' AAGTCGGCCATCTTCTTCCT 3'
SSR size
151
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2B
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2278-2B
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