GrainGenes Marker Report: Xcfa2257-7B
Locus
Xcfa2257-7B
Type
Microsatellite
Map
Kofa/UC1113-7B 199.3
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Probe
CFA2257
Possible Orthologs
Xcfa2257 Xcfa2257-7A cfa2257 cfa2257-7A cfa2257-7B
Probe for Locus
CFA2257
Locus
Xcfa2257-7A
[ Show all 6 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)28 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' GATACAATAGGTGCCTCCGC 3'
Reverse 5' CCATTATGTAAATGCTTCTGTTTGA 3'
SSR size
167
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2257-7B
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