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GrainGenes Marker Report: Xcfa2257

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Locus	Xcfa2257
Type	Microsatellite
Map	Ta-NxW-7A 295.8 Wheat, UC1110 x PI610750 7A 202.6 Wheat, UC1110 x PI610750 7A 203.1
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	CFA2257
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xcfa2257-7A Xcfa2257-7B cfa2257 cfa2257-7A cfa2257-7B
Probe for Locus	CFA2257
Locus	Xcfa2257-7A [ Show all 6 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)28 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GATACAATAGGTGCCTCCGC 3' Reverse 5' CCATTATGTAAATGCTTCTGTTTGA 3'
SSR size	167
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7A
Source Species	Triticum urartu