GrainGenes Marker Report: Xcfa2256-4A
Locus
Xcfa2256-4A
Type
Microsatellite
Chromosome
4A
Map
Chinese_Spring_Deletion_SSR_4A -0.88 Louise/Penawawa-4A 97.9 Ta-Courtot/CS-GPW-4A 38.5 Ta-SSR-2004-4A 10
[ Show Nearby Loci ]
Breakpoint Interval
4AS3-0.76-1.00
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa Wheat, Physical, SSR
Probe
CFA2256
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
cfa2256 cfa2256-4A
Probe for Locus
CFA2256
Locus
Xcfa2256-4A cfa2256 cfa2256-4A
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)29 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GGTAATATTCAGGTTACCGCACA 3'
Reverse 5' GGTAAAGTTATAAATTGTTGTGGGC 3'
SSR size
171
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2256-4A
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