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GrainGenes Marker Report: Xcfa2241-1B

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Locus	Xcfa2241-1B
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_1B -0.67 P91193D1/P92201D5-1B 58.7 Ta-Synthetic/Opata-GPW-1B 33.3
	[ Show Nearby Loci ]
Breakpoint Interval	1BS10-0.50-0.84
Map Data	Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe	CFA2241
Possible Orthologs	Xcfa2241
Probe for Locus	CFA2241
Locus	Xcfa2241-1B Xcfa2241
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)21 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TTGGCCATCAGGCTCTAGTT 3' Reverse 5' GTGATGCTGTTCTCAAGCCA 3'
SSR size	225
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1B
Source Species	Triticum urartu