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GrainGenes Marker Report: Xcfa2240

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Locus	Xcfa2240
Type	Microsatellite
Map	Ta-NxW-7A 302.8 Wheat_Fhb7_2020 9.856
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Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	CFA2240
Possible Orthologs	Xcfa2240-7A
Probe for Locus	CFA2240
Locus	Xcfa2240-7A Xcfa2240
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TA)n Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TGCAGCATGCATTTTAGCTT 3' Reverse 5' TGCCGCACTTATTTGTTCAC 3'
SSR size	280
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7A
Source Species	Triticum urartu