GrainGenes Marker Report: Xcfa2234-3A
Locus
Xcfa2234-3A
Type
Microsatellite
Chromosome
3A
Map
CS/SQ1-3A 74.3 Kofa/UC1113-3A 41.3 Ta-Courtot/CS-GPW-3A 88.7 Ta-SSR-2004-3A 51.6
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW
Probe
CFA2234
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2234 cfa2234 cfa2234-3A
Probe for Locus
CFA2234
Locus
Xcfa2234-3A Xcfa2234 cfa2234 cfa2234-3A
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)17 Regularity of motif is 0
Type
PCR SSR
PCR primers
Forward 5' AATCTGACCGAACAAAATCACA 3'
Reverse 5' TCGGAGAGTATTAGAACAGTGCC 3'
SSR size
151
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2234-3A
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